Methods 18 mer nucleic acid was synthesized and complementary to the translation initiation site of mRNA of dipterex resistant mosquitoes. The ODNs were annealed to the corresponding mRNA molecules and they were added to rabbit reticulocyte cell free system. The translation products were analyzed by SDS PAGE. 方法人工合成互补于抗性库蚊酯酶mRNA翻译起始点18碱基,与淡色库蚊mRNA退火后加入无细胞翻译体系,进行体外翻译,翻译产物用SDS聚丙烯酰胺凝胶电泳进行分析。
Methods Three 20 mers phosphorothioate oligodeoxynucleotides ( ODNs) were synthesized: ( 1) Cyclin D1 antisense ODN ( AS ODN) targeting the initiation site of translation in cyclin D1 mRNA; 方法合成针对细胞周期素D1mRNA翻译起始部位序列的20bp硫代磷酸反向寡脱氧核苷酸(AsODN)。